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Cancer Research
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RNAシーケンシング用の3つの微分発現解析方法:リンマ、エッジャー、DESeq2
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Cancer Research
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JoVE Journal
Cancer Research
Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2
Please note that all translations are automatically generated.
Click here for the English version.
RNAシーケンシング用の3つの微分発現解析方法:リンマ、エッジャー、DESeq2
DOI:
10.3791/62528-v
•
10:10 min
•
September 18, 2021
•
Shiyi Liu*
1
,
Zitao Wang*
1
,
Ronghui Zhu
,
Feiyan Wang
,
Yanxiang Cheng
,
Yeqiang Liu
1
Department of Obstetrics and Gynecology
,
Renmin Hospital of Wuhan University
,
2
Department of Pathology, Shanghai Skin Disease Hospital
,
Tongji University School of Medicine
Chapters
00:08
Introduction
01:54
I. Download and preprocess the data
05:12
II. RNA‐seq differential expression analysis through “limma”
08:53
III. RNA‐seq differential expression analysis through
“edgeR”
11:34
IV. RNA‐seq differential expression analysis through “DESeq2”
13:51
V. Venn diagram
14:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
RNAシーケンシングの微分発現解析方法の詳細なプロトコルが提供されました: リンマ, エッジ, DESeq2.
Tags
RNA Sequencing
Differential Expression Analysis
Limma
EdgeR
DESeq2
Cholangiocarcinoma
Cancer
Gene Expression
Ensemble Gene ID
Gene Symbol
Low-expressed Genes
Design Matrix
DGEList
Normalization
Linear Model
T-statistic
F-statistic
Log-odds
Log2 Fold Change
FDR
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