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Journal
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Cancer Research
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RNA 测序的三种微分表达分析方法:利马、边缘、DESeq2
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Cancer Research
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JoVE Journal
Cancer Research
Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2
Please note that all translations are automatically generated.
Click here for the English version.
RNA 测序的三种微分表达分析方法:利马、边缘、DESeq2
DOI:
10.3791/62528-v
•
10:10 min
•
September 18, 2021
•
Shiyi Liu*
1
,
Zitao Wang*
1
,
Ronghui Zhu
,
Feiyan Wang
,
Yanxiang Cheng
,
Yeqiang Liu
1
Department of Obstetrics and Gynecology
,
Renmin Hospital of Wuhan University
,
2
Department of Pathology, Shanghai Skin Disease Hospital
,
Tongji University School of Medicine
Chapters
00:08
Introduction
01:54
I. Download and preprocess the data
05:12
II. RNA‐seq differential expression analysis through “limma”
08:53
III. RNA‐seq differential expression analysis through
“edgeR”
11:34
IV. RNA‐seq differential expression analysis through “DESeq2”
13:51
V. Venn diagram
14:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提供了RNA测序的微分表达分析方法的详细协议:伽利马、EdgeR、DESeq2。
Tags
RNA Sequencing
Differential Expression Analysis
Limma
EdgeR
DESeq2
Cholangiocarcinoma
Cancer
Gene Expression
Ensemble Gene ID
Gene Symbol
Low-expressed Genes
Design Matrix
DGEList
Normalization
Linear Model
T-statistic
F-statistic
Log-odds
Log2 Fold Change
FDR
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