00:44Automated Visualization Protocol for Spatial Proteomics Assays
06:02Spatial Profiling Platform Protocol for Proteomics Assay
06:47ROI Selection for Spatial Transcriptomics Assays
07:10Results: Application of the Automated TSA Based Visualization Protocol in Combination with Spatial Proteomics Assays and Detection of Small ROIs in the Spatial Transcriptomics Protocol
Here, we describe a protocol for fine-tuning regions of interest (ROIs) for Spatial Omics technologies to better characterize the tumor microenvironment and identify specific cell populations. For proteomics assays, automated customized protocols can guide ROI selection, while transcriptomics assays can be fine-tuned utilizing ROIs as small as 50 µm.