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使用两步PCR和下一代16S rRNA基因测序进行微生物群分析

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Biology

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JoVE Journal Biology

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Please note that all translations are automatically generated. Click here for the English version.

使用两步PCR和下一代16S rRNA基因测序进行微生物群分析

DOI:

10.3791/59980-v

•

11:22 min

•

October 15, 2019

•

Shailesh K. Shahi, Kasra Zarei, Natalya V. Guseva, Ashutosh K. Mangalam^2,3,4

¹Department of Pathology,University of Iowa, ²Medical Scientist Training Program,University of Iowa, ³Graduate Program in Immunology,University of Iowa, ⁴Graduate Program in Molecular Medicine,University of Iowa

Chapters

00:04Title
01:25Extraction of DNA from Fecal Sample
02:2916S rRNA Gene Amplification (PCR1) and DNA Library Construction (PCR2)
03:57Clean-up of Indexed PCR (PCR2) and Quantification
06:03Sequence Analysis
07:49Results: Quality Profile and Statistical Analysis
09:29Conclusion

Summary

Automatic Translation

这里描述的是使用16S rRNA基因组测序和利用免费工具进行分析的微生物群分析的简化标准操作程序。该协议将帮助那些对微生物群领域较新的研究人员，以及那些需要更新方法以更高分辨率实现细菌分析的研究人员。

Tags

Microbiome Microbiota 16S RRNA Gene Sequencing Next-generation Sequencing Fecal Sample DNA Extraction PCR Gut Microbiome Microbial Profiling

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