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A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
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A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

DOI:
10.3791/62354-v

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05:34 min

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April 15, 2021

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, , , , , ,
1Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences,University of Florida, 2U. S. Fish and Wildlife Service, Pacific Islands Fish and Wildlife Office, 3Department of Entomology and Nematology,University of California Davis

Chapters

  • 00:04Introduction
  • 00:46Sample Disruption
  • 01:31DNA Extraction
  • 03:49Results: DNA Quality and Cost Per Sample
  • 04:38Conclusion

Summary

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Described here is a DNA extraction protocol using magnetic beads to produce high quality DNA extractions from mosquitoes. These extractions are suitable for a downstream next-generation sequencing approach.

Tags

Magnetic Bead-based DNA ExtractionMosquito DNANext-generation SequencingBudget-friendly ProtocolResource-limited LabsDiagnosticResearchHigh-throughput SequencingProteinase KMagnetic Bead Master MixWashing BufferElution Buffer

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