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Journal
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Medicine
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使用DESI-MSI鉴定中药空间代谢组中代谢物的可视化
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Medicine
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JoVE Journal
Medicine
Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI
Please note that all translations are automatically generated.
Click here for the English version.
使用DESI-MSI鉴定中药空间代谢组中代谢物的可视化
DOI:
10.3791/64912-v
•
08:02 min
•
December 16, 2022
•
Binjie Xu
4
,
Lirun Chen
,
Fengqi Lv
,
Yuan Pan
,
Xing Fu
,
Zhaoqing Pei
2
1
Innovative Institute of Chinese Medicine and Pharmacy
,
Chengdu University of Traditional Chinese Medicine
,
2
Tianfu Chinese Medicine Innovation Harbour
,
Chengdu University of Traditional Chinese Medicine
,
3
School of Medical Technology
,
Chengdu University of Traditional Chinese Medicine
,
4
China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd.
Chapters
00:04
Introduction
01:02
Sample Preparation
01:55
Installation of Desorption Electrospray Ionization (DESI) Unit
04:02
Desorption Electrospray Ionization‐Mass Spectrometry (DESI‐MS) Image Acquisition
05:50
Processing DESI‐MSI Data and Visualization
06:55
Results: Mass Spectrometry Imaging of Root and Whole Leaf Sections
07:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究提出了一系列从工厂制备DESI-MSI样品的方法,并详细描述了DESI组件安装、MSI数据采集和处理的过程。该协议可以应用于获取植物空间代谢组信息的几种条件。
Tags
Spatial Metabolomics
DESI-MSI
Plant Sample Preparation
Cryosectioning
Imprinting
Salvia Miltiorrhiza
Sample Freezing
Vacuum Drying
Matrix Application
ESI Mode
DESI Unit Setup
Solvent Ionization
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