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使用DESI-MSI鉴定中药空间代谢组中代谢物的可视化
JoVE Journal
Medicine
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JoVE Journal Medicine
Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI
DOI:

08:02 min

December 16, 2022

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Chapters

  • 00:04Introduction
  • 01:02Sample Preparation
  • 01:55Installation of Desorption Electrospray Ionization (DESI) Unit
  • 04:02Desorption Electrospray Ionization‐Mass Spectrometry (DESI‐MS) Image Acquisition
  • 05:50Processing DESI‐MSI Data and Visualization
  • 06:55Results: Mass Spectrometry Imaging of Root and Whole Leaf Sections
  • 07:31Conclusion

Summary

Automatic Translation

本研究提出了一系列从工厂制备DESI-MSI样品的方法,并详细描述了DESI组件安装、MSI数据采集和处理的过程。该协议可以应用于获取植物空间代谢组信息的几种条件。

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