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単球/マクロファージにおける腫瘍微小環境の採用を検討する癌細胞株における遺伝子発現の条件付きのノックダウン
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Cancer Research
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
Please note that all translations are automatically generated.
Click here for the English version.
単球/マクロファージにおける腫瘍微小環境の採用を検討する癌細胞株における遺伝子発現の条件付きのノックダウン
DOI:
10.3791/56333-v
•
10:59 min
•
November 23, 2017
•
Marta H. Kubala
2
,
Yves A. DeClerck
2,3
1
Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics
,
University of Southern California
,
2
The Saban Research Institute of Children’s Hospital Los Angeles
,
3
Department of Biochemistry and Molecular Medicine
,
University of Southern California
Chapters
00:05
Title
01:09
Generation of Lentivirus Particles
02:34
Generation of Stably Transfected Lines
04:55
Isolation of Monocytes from Human Blood
08:00
Boyden Chamber Migration Assay
09:25
Results: PAI-1 Promotes Macrophage Migration
10:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、単球/マクロファージにおける腫瘍微小環境の募集に腫瘍細胞由来タンパク質の役割を研究するため特に癌細胞とその後続の使用で機能するテトにシステムを設定するためのスキームとして機能します。
Tags
Conditional Gene Knockdown
Cancer Cell Lines
Monocyte Recruitment
Macrophage Recruitment
Tumor Microenvironment
Lentiviral Transduction
HEK-293 Cells
HCT-116 Cells
MDA-MB-231 Cells
Monocyte Purification
Puromycin Selection
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