JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
肿瘤细胞系基因表达的条件性击倒研究单核/巨噬细胞在微环境中的招募
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Cancer Research
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
Please note that all translations are automatically generated.
Click here for the English version.
肿瘤细胞系基因表达的条件性击倒研究单核/巨噬细胞在微环境中的招募
DOI:
10.3791/56333-v
•
10:59 min
•
November 23, 2017
•
Marta H. Kubala
2
,
Yves A. DeClerck
2,3
1
Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics
,
University of Southern California
,
2
The Saban Research Institute of Children’s Hospital Los Angeles
,
3
Department of Biochemistry and Molecular Medicine
,
University of Southern California
Chapters
00:05
Title
01:09
Generation of Lentivirus Particles
02:34
Generation of Stably Transfected Lines
04:55
Isolation of Monocytes from Human Blood
08:00
Boyden Chamber Migration Assay
09:25
Results: PAI-1 Promotes Macrophage Migration
10:24
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本议定书作为一个计划, 建立一个功能性的癌症细胞系的系统, 并随后使用, 特别是研究肿瘤细胞源性蛋白质在招募单核/巨噬细胞到肿瘤微环境的作用。
Tags
Conditional Gene Knockdown
Cancer Cell Lines
Monocyte Recruitment
Macrophage Recruitment
Tumor Microenvironment
Lentiviral Transduction
HEK-293 Cells
HCT-116 Cells
MDA-MB-231 Cells
Monocyte Purification
Puromycin Selection
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
肺肿瘤细胞招募分析
该<em>果蝇</em>成虫盘瘤模型:基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克
线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡
的
实验研究
体内
在肿瘤微环境中 pH 值、
p
O
2
、氧化还原状态、磷酸盐和谷胱甘肽浓度的 EPR 评价
CRISPR 指南对小鼠前列腺病毒传递的基因改变
活体细胞成像技术研究胶质瘤脑干细胞的迁移和侵袭
建立过度表达 dr3 的细胞系, 评估抗有丝分裂治疗的凋亡反应
分析肿瘤基因表达因子与CorExplorer门户网站
固体肿瘤细胞系侧面人口分析
结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因
Read Article