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Ratón Sistema Cultura Hígado Fetal de diseccionar las funciones de genes objetivo en las primeras y últimas etapas de la Terminal de la eritropoyesis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
DOI:

06:40 min

September 09, 2014

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Chapters

  • 00:05Title
  • 01:21Purification of Fetal Liver Erythroblasts
  • 02:32Purification of TER119 Negative Erythroblasts
  • 03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
  • 04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
  • 06:08Conclusion

Summary

Automatic Translation

Presentamos un ratón sistema de cultivo in vitro fetal eritroblasto hígado que disecciona las etapas tempranas y tardías de la eritropoyesis terminal. Este sistema facilita el análisis funcional de genes específicos en diferentes etapas de desarrollo.

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