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マウス胎児肝臓培養システムは、ターミナル赤血球の初期および後期段階での標的遺伝子の機能を分析する
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
DOI:

06:40 min

September 09, 2014

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Chapters

  • 00:05Title
  • 01:21Purification of Fetal Liver Erythroblasts
  • 02:32Purification of TER119 Negative Erythroblasts
  • 03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
  • 04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
  • 06:08Conclusion

Summary

Automatic Translation

私たちは、端末赤血球生成の初期および後期段階を解剖インビトロマウス胎児肝臓赤芽球培養系を提示。このシステムは、異なる発生段階における特定の遺伝子の機能解析を容易にします。

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