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Muis foetale lever Cultuur System Doel Gene functies ontleden in de vroege en late stadia van Terminal Erytropoëse
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
DOI:

06:40 min

September 09, 2014

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Chapters

  • 00:05Title
  • 01:21Purification of Fetal Liver Erythroblasts
  • 02:32Purification of TER119 Negative Erythroblasts
  • 03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
  • 04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
  • 06:08Conclusion

Summary

Automatic Translation

We geven een in vitro muizen foetale lever erytroblast kweeksysteem dat de vroege en late stadia van terminal erytropoëse ontleedt. Dit systeem vergemakkelijkt de functionele analyse van specifieke genen in verschillende ontwikkelingsstadia.

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