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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
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Immunology and Infection
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JoVE Journal Immunology and Infection
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

DOI:
10.3791/51522-v

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10:48 min

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May 15, 2014

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, , ,
1Department of Microbiology,Boston University School of Medicine

Chapters

  • 00:05Title
  • 01:29Multi-stage Reporter Viruses
  • 02:27Plate Cells
  • 05:08Fixing Cells
  • 06:13Quantify Virus Growth
  • 07:29Alternative Protocol: Kinetic Plate Assays
  • 08:29Effect of Poxvirus Inhibitors on TrpV Expression
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.

Tags

PoxvirusVaccinia VirusReporter VirusGene ExpressionFluorescent ProteinViral ReplicationHigh-throughputAntiviral DiscoveryVirus-host InteractionEvolutionary Biology

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