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Journal
/
Biology
/
CLON-Gおよび
in vitro
自然死アッセイによる好中球の寿命延長
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Biology
Neutrophil Lifespan Extension with CLON-G and an
In Vitro
Spontaneous Death Assay
Please note that all translations are automatically generated.
Click here for the English version.
CLON-Gおよび
in vitro
自然死アッセイによる好中球の寿命延長
DOI:
10.3791/65132-v
•
05:52 min
•
May 12, 2023
•
Yuping Fan*
1,2
,
Yan Teng*
3
,
Fu tong Liu*
4
,
Fengxia Ma
2
,
Alan Y. Hsu
,
Sizhou Feng
2
,
Hongbo R. Luo
1
State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital
,
Chinese Academy of Medical Sciences & Peking Union Medical College
,
2
Tianjin Institutes of Health Science
,
3
Sichuan Provincial People’s Hospital
,
University of Electronic Science and Technology of China
,
4
Haihe Laboratory of Cell Ecosystem
,
Tianjin Medical University
,
5
Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children’s Hospital
,
Dana-Farber /Harvard Cancer Center
Chapters
00:04
Introduction
00:44
Neutrophil Lifespan Extension with CLON‐G
02:13
In Vitro Spontaneous Death Assay of Neutrophil
04:06
Results: Effect of CLON‐G Medium on Neutrophil Viability
05:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、好中球の寿命を5日以上に延長するためのCLON-Gの調製を詳述し、フローサイトメトリーと共焦点蛍光顕微鏡で好中球死を評価するための信頼できる手順を提供します。
Tags
Neutrophil Lifespan
CLON-G
In Vitro Spontaneous Death Assay
Neutrophil Preservation
Neutrophil Transportation
Neutrophil Gene Manipulation
Flow Cytometry
Fluorescent Imaging
Cell Culture
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