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1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem,Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children’s Hospital,Dana-Farber /Harvard Cancer Center
Chapters
- 00:04Introduction
- 00:44Neutrophil Lifespan Extension with CLON‐G
- 02:13In Vitro Spontaneous Death Assay of Neutrophil
- 04:06Results: Effect of CLON‐G Medium on Neutrophil Viability
- 05:11Conclusion
Questo protocollo descrive in dettaglio la preparazione di CLON-G per estendere la durata della vita dei neutrofili a più di 5 giorni e fornisce una procedura affidabile per valutare la morte dei neutrofili con citometria a flusso e microscopia a fluorescenza confocale.
