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Biology
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通过 CLON-G 和
体外
自发死亡测定延长中性粒细胞寿命
JoVE Journal
Biology
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JoVE Journal
Biology
Neutrophil Lifespan Extension with CLON-G and an
In Vitro
Spontaneous Death Assay
Please note that all translations are automatically generated.
Click here for the English version.
通过 CLON-G 和
体外
自发死亡测定延长中性粒细胞寿命
DOI:
10.3791/65132-v
•
05:52 min
•
May 12, 2023
•
Yuping Fan*
1,2
,
Yan Teng*
3
,
Fu tong Liu*
4
,
Fengxia Ma
2
,
Alan Y. Hsu
,
Sizhou Feng
2
,
Hongbo R. Luo
1
State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital
,
Chinese Academy of Medical Sciences & Peking Union Medical College
,
2
Tianjin Institutes of Health Science
,
3
Sichuan Provincial People’s Hospital
,
University of Electronic Science and Technology of China
,
4
Haihe Laboratory of Cell Ecosystem
,
Tianjin Medical University
,
5
Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children’s Hospital
,
Dana-Farber /Harvard Cancer Center
Chapters
00:04
Introduction
00:44
Neutrophil Lifespan Extension with CLON‐G
02:13
In Vitro Spontaneous Death Assay of Neutrophil
04:06
Results: Effect of CLON‐G Medium on Neutrophil Viability
05:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议详细介绍了CLON-G的制备,以将中性粒细胞寿命延长至5天以上,并为使用流式细胞术和共聚焦荧光显微镜评估中性粒细胞死亡提供了可靠的程序。
Tags
Neutrophil Lifespan
CLON-G
In Vitro Spontaneous Death Assay
Neutrophil Preservation
Neutrophil Transportation
Neutrophil Gene Manipulation
Flow Cytometry
Fluorescent Imaging
Cell Culture
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