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1Mechanobiology Institute (MBI),National University of Singapore, 2Biomedical Engineering Department,National University of Singapore, 3Department of Biological Sciences,National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine,National University of Singapore
Chapters
- 00:04Introduction
- 00:49PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer
- 02:19Coverslip Substrate Preparation
- 03:34Transferring the Textured Film to the Final Substrate
- 04:33Cell Seeding, Release, and Collection of the Organoids
- 06:29Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation
- 07:00Conclusion
В этой статье представлен протокол изготовления нового типа культуральной подложки с сотнями микроконтейнеров намм2, в котором органоиды можно культивировать и наблюдать с помощью микроскопии высокого разрешения. Протоколы посева клеток и иммуноокрашивания также детализированы.
