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用于类器官培养和 3D 成像的高通量平台
JoVE Journal
Biology
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JoVE Journal
Biology
A High-Throughput Platform for Culture and 3D Imaging of Organoids
Please note that all translations are automatically generated.
Click here for the English version.
用于类器官培养和 3D 成像的高通量平台
DOI:
10.3791/64405-v
•
07:42 min
•
October 14, 2022
•
Gianluca Grenci
2
,
Florian Dilasser
,
Saburnisha Binte Mohamad Raffi
,
Marion Marchand
,
Mona Suryana
,
Geetika Sahni
,
Virgile Viasnoff
3,4
,
Anne Beghin
5
1
Mechanobiology Institute (MBI)
,
National University of Singapore
,
2
Biomedical Engineering Department
,
National University of Singapore
,
3
Department of Biological Sciences
,
National University of Singapore
,
4
IRL 3639 CNRS
,
5
Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine
,
National University of Singapore
Chapters
00:04
Introduction
00:49
PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer
02:19
Coverslip Substrate Preparation
03:34
Transferring the Textured Film to the Final Substrate
04:33
Cell Seeding, Release, and Collection of the Organoids
06:29
Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation
07:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种新型培养基质的制造方案,每mm
2
具有数百个微容器,其中可以使用高分辨率显微镜培养和观察类器官。还详细介绍了细胞接种和免疫染色方案。
Tags
High-throughput
Organoid Culture
3D Imaging
Microtexture Cell Culture Dish
PDMS Mold
UV Curable Adhesive
Spin Coating
Plasma Treatment
Biomedical Applications
Drug Screening
Cancer Research
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