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Optimisation du modèle murin d’occlusion veineuse rétinienne pour limiter la variabilité
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1Department of Pathology & Cell Biology; Vagelos College of Physicians and Surgeons,Columbia University, 2Department of Molecular Pharmacology and Therapeutics; Vagelos College of Physicians and Surgeons,Columbia University, 3Department of Neurology; Vagelos College of Physicians and Surgeons,Columbia University, 4The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain; Vagelos College of Physicians and Surgeons,Columbia University
Chapters
- 00:05Introduction
- 00:45Laser Setup
- 02:05Mouse Tail Vein Injection of Rose Bengal
- 02:54Occlusion of Major Veins
- 04:07Establishing the Number of Veins Occluded at Day 0 and Aftercare
- 04:40Results: Analysis of Types and Timelines of Photo-Occlusion, Baseline and Experimental Laser Output Measurement, Ophthalmic Pathologies, and Quantification of OCT Images
- 06:37Conclusion
Ici, nous décrivons un protocole optimisé pour l’occlusion veineuse rétinienne à l’aide de rose bengale et un système de microscope d’imagerie rétinienne guidé par laser avec des recommandations pour maximiser sa reproductibilité dans les souches génétiquement modifiées.
