Journal
/
/
Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability
DOI:

07:23 min

August 06, 2021

, , , ,

Chapters

  • 00:05Introduction
  • 00:45Laser Setup
  • 02:05Mouse Tail Vein Injection of Rose Bengal
  • 02:54Occlusion of Major Veins
  • 04:07Establishing the Number of Veins Occluded at Day 0 and Aftercare
  • 04:40Results: Analysis of Types and Timelines of Photo-Occlusion, Baseline and Experimental Laser Output Measurement, Ophthalmic Pathologies, and Quantification of OCT Images
  • 06:37Conclusion

Summary

Automatic Translation

Here, we describe an optimized protocol for retinal vein occlusion using rose bengal and a laser-guided retinal imaging microscope system with recommendations to maximize its reproducibility in genetically modified strains.

Related Videos

Read Article