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Cancer Research
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通过恶性周围神经鞘肿瘤细胞中絮状等位基因的
离体
消融来定义肿瘤发生的基因功能
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Defining Gene Functions in Tumorigenesis by
Ex vivo
Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
Please note that all translations are automatically generated.
Click here for the English version.
通过恶性周围神经鞘肿瘤细胞中絮状等位基因的
离体
消融来定义肿瘤发生的基因功能
DOI:
10.3791/62740-v
•
09:37 min
•
August 25, 2021
•
Jody Fromm Longo
,
Stephanie N. Brosius
3
,
Steven L. Carroll
1
Department of Pathology and Laboratory Medicine
,
Medical University of South Carolina
,
2
Department of Pediatrics and Neurology
,
Perelman School of Medicine of the University of Pennsylvania
,
3
Division of Child Neurology
,
Children’s Hospital of Philadelphia
Chapters
00:05
Introduction
00:52
Tumor Dissection and Staining
01:46
Ex Vivo Ablation of Floxed
Erbb4
Alleles in MPNST Cells
03:52
Proliferation Assays in MPNST Cells with Ablated
Erbb4
Alleles
04:23
RNA-Seq Analyses and Gene Identification with Altered Expression by
Erbb4
Loss
06:04
Orthotopic Allograft of MPNST Cells with Ablated
Erbb4
Alleles and
Erbb4
Ablation Effects Analysis
07:10
Results: Effect of Adenovirus Transduction on MPNST Cells and Allografts
09:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一种方案,用于在基因工程小鼠模型衍生
的肿瘤中
执行胚胎在体内致命的基因敲除,然后评估敲除对肿瘤生长,增殖,存活,迁移,侵袭以及
体外
和
体内
转录组的影响。
Tags
Gene Function
Tumorigenesis
Ex Vivo Ablation
Floxed Alleles
Malignant Peripheral Nerve Sheath Tumor
Genetically Engineered Mouse Model
Adenoviral Infection
Early Passage Culture
Immunohistochemical Staining
Neuregulin-1 Beta
Forskolin
EGFP
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