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Culture and Imaging of Ex Vivo Organotypic Pseudomyxoma Peritonei Tumor Slices from Resected Human Tumor Specimens
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Chapters
- 00:04Introduction
- 00:48Preparation of Human Pseudomyxoma Peritonei (PMP) Tissues for Tissue Processing and Culture
- 03:07Confocal Imaging of Living Human Tissue Slices
- 03:51Disassociation of Living Slices for Flow Cytometry
- 05:23Pharmacological Intervention Using Living Tissue Slices for Viability and Proliferation Analysi
- 06:31Results: Real-Time Visualization and Interrogation of Tumor Using Patient-Derived Ex Vivo Organotypic Slices
- 08:43Conclusion
We describe a protocol for the production, culture, and visualization of human cancers, which have metastasized to the peritoneal surfaces. Resected tumor specimens are cut using a vibratome and cultured on permeable inserts for increased oxygenation and viability, followed by imaging and downstream analyses using confocal microscopy and flow cytometry.
