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Análise de transcriptome de alto rendimento para investigar interações host-pathogen
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
DOI:

14:58 min

March 05, 2022

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Chapters

  • 00:07Introduction
  • 01:15Docker Desktop Installation
  • 04:25Quality Control
  • 05:10Mapping and Annotation Steps
  • 05:51Differentially Expressed Genes Process
  • 06:10Transfer Data to the Personal Computer
  • 12:02Representative Results
  • 14:22Conclusions

Summary

Automatic Translation

O protocolo aqui apresentado descreve um pipeline completo para analisar dados de transcriptome de sequenciamento de RNA desde leituras brutas até análises funcionais, incluindo etapas de controle de qualidade e pré-processamento para abordagens analíticas estatísticas avançadas.

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