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Bepaling van totale lipide- en lipideklassen in mariene monsters
JoVE Journal
Environment
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JoVE Journal Environment
Determination of Total Lipid and Lipid Classes in Marine Samples
DOI:

14:59 min

December 11, 2021

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Chapters

  • 00:04Introduction
  • 00:32Filtration Procedure for Seawater Dissolved and Particulate Lipids
  • 01:53Liquid-Liquid Extraction of Seawater or Liquid Samples
  • 03:23Extraction Protocol for Solids (Modified Folch et al. Extraction)
  • 06:17Developing and Conditioning Sequences for Separation of Aquatic Lipid Classes by Rod TLC
  • 10:11FAME Derivatization with H2SO4 in MeOH
  • 13:17Representative Results
  • 14:30Conclusion

Summary

Automatic Translation

Dit protocol is voor de bepaling van lipiden in zeewater en biologische specimens. Lipiden in filtraten worden geëxtraheerd met chloroform of mengsels van chloroform en methanol in het geval van vaste stoffen. Lipideklassen worden gemeten door staaf dunnelaagchromatografie met vlamionisatiedetectie en hun som geeft het totale lipidengehalte.

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