Journal
/
/
海洋样品中总脂质和脂质类别的测定
JoVE Journal
Environment
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Determination of Total Lipid and Lipid Classes in Marine Samples
DOI:

14:59 min

December 11, 2021

,

Chapters

  • 00:04Introduction
  • 00:32Filtration Procedure for Seawater Dissolved and Particulate Lipids
  • 01:53Liquid-Liquid Extraction of Seawater or Liquid Samples
  • 03:23Extraction Protocol for Solids (Modified Folch et al. Extraction)
  • 06:17Developing and Conditioning Sequences for Separation of Aquatic Lipid Classes by Rod TLC
  • 10:11FAME Derivatization with H2SO4 in MeOH
  • 13:17Representative Results
  • 14:30Conclusion

Summary

Automatic Translation

该协议用于测定海水和生物标本中的脂质。滤液中的脂质在固体的情况下用氯仿或氯仿和甲醇的混合物提取。脂类通过棒状薄层色谱法和火焰电离检测来测量,其总和给出了总脂质含量。

Related Videos

Read Article