JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Environment
/
海洋样品中总脂质和脂质类别的测定
JoVE Journal
Environment
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Environment
Determination of Total Lipid and Lipid Classes in Marine Samples
Please note that all translations are automatically generated.
Click here for the English version.
海洋样品中总脂质和脂质类别的测定
DOI:
10.3791/62315-v
•
14:59 min
•
December 11, 2021
•
C. C. Parrish
,
J. S. Wells
1
Department of Ocean Sciences and CREAIT Network
,
Memorial University of Newfoundland
Chapters
00:04
Introduction
00:32
Filtration Procedure for Seawater Dissolved and Particulate Lipids
01:53
Liquid-Liquid Extraction of Seawater or Liquid Samples
03:23
Extraction Protocol for Solids (Modified Folch et al. Extraction)
06:17
Developing and Conditioning Sequences for Separation of Aquatic Lipid Classes by Rod TLC
10:11
FAME Derivatization with H
2
SO
4
in MeOH
13:17
Representative Results
14:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议用于测定海水和生物标本中的脂质。滤液中的脂质在固体的情况下用氯仿或氯仿和甲醇的混合物提取。脂类通过棒状薄层色谱法和火焰电离检测来测量,其总和给出了总脂质含量。
Tags
Total Lipid
Lipid Classes
Marine Samples
Liquid-liquid Extraction
Non-polar Organic Solvents
Dissolved And Particulate Fractions
Filtration
Chloroform Extraction
Lipid-clean
Seawater
Biological Specimens
Lipid Enrichment
Salts And Protein Removal
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
一种非侵入性的方法<em>原位</em>交配成功的女美国龙虾(测定<em>美洲螯龙虾</em>)
了鱼喂食实验室生物测定,从海洋生物的组织评估次生代谢产物的Antipredatory活动
VacuSIP,对改进的方法INEX<em>原位</em>颗粒物与测量溶解的化合物加工后主动悬挂给料机
用于表征土壤微生物的脂质提取和分析方法在许多样品的实验中
原位
含甲烷沉积物中自黄铁矿的制备及应用模拟器硫同位素分析
海洋浮游生物和悬浮颗粒的超洁净多层采集装置
基于图像的海底无脊椎动物及其栖息地调查方法大西洋扇贝落相机调查
藤壶
Balanus improvisus
作为海洋模型的培养和基因表达
就地
石蜡镶嵌成人珊瑚样品的杂交技术
Tigriopus
桡足的个体培养及其交配行为的定量分析
Read Article