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Cancer Research
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单核RNA-seq和ATAC-seq的新鲜冷冻脑肿瘤的核隔离
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Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
Please note that all translations are automatically generated.
Click here for the English version.
单核RNA-seq和ATAC-seq的新鲜冷冻脑肿瘤的核隔离
DOI:
10.3791/61542-v
•
06:22 min
•
August 25, 2020
•
Ashwin Narayanan
,
Enrique Blanco-Carmona
,
Engin Demirdizen
,
Xueyuan Sun
3
,
Christel Herold-Mende
,
Matthias Schlesner
,
Sevin Turcan
1
Neurology Clinic and National Center for Tumor Diseases
,
University Hospital Heidelberg
,
2
Bioinformatics and Omics Data Analytics
,
German Cancer Research Center (DKFZ)
,
3
Clinical Cooperation Unit Neurooncology
,
German Cancer Research Center (DKFZ)
,
4
Division of Experimental Neurosurgery, Department of Neurosurgery
,
University of Heidelberg, INF 400, Heidelberg, Germany
Chapters
00:04
Introduction
01:01
Tissue Dissection and Dissociation
03:20
Gradient Centrifugation and Isolation of Nuclei
04:56
Results: Comparison of UMIs and Genes Obtained from Different Human Brain snRNA-seq Datasets
05:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肿瘤内异质性是肿瘤的内在特征,包括胶质瘤。我们开发了一个简单而高效的协议,利用缓冲和梯度离心的组合,将单核从新鲜冷冻胶质瘤组织中分离出来,用于单核RNA和ATAC测序研究。
Tags
Keyword Extraction:Nuclei Isolation
Fresh Frozen Brain Tumors
Single-nucleus RNA-seq
ATAC-seq
Tumor Evolution
Tumor Composition
Nuclei Preparation
Transcriptional Studies
Epigenetic Studies
Archival Frozen Tumors
Nuclei Lysis Buffer
Nuclear Membrane
Nuclei Centrifugation
Nuclei Resuspension
HB Buffer
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