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Valutazione della morte cellulare utilizzando un surnatante privo di cellule di probiotici in colture sferoidali tridimensionali di cellule tumorali del colon-retto
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1Microbiological Analysis Team, Group for Biometrology,Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy,Chungnam National University (CNU), 3Stem Cell Research Center,Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection,Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science,University of Science & Technology (UST)
Chapters
- 00:04Introduction
- 00:34Bacterial Cell Culture and Lactobacillus Cell-Free Supernatant (LCFS) Preparation
- 02:18Colorectal Cancer Cell Line Preparation
- 03:12Spheroid Formation
- 03:493D colorectal Cancer Cell LCFS Treatment
- 04:26Results: Representative Effects of LCFS Co-Culture on Spheroid Formation and Viability
- 05:22Conclusion
Qui vengono presentati i metodi per comprendere gli effetti anti-cancro del surnatante senza cellule di Lactobacillus (LCFS). Le linee cellulari del cancro del colon-retto mostrano decessi cellulari quando trattati con LCFS in colture 3D. Il processo di generazione degli sferoidi può essere ottimizzato a seconda dell'impalcatura e i metodi di analisi presentati sono utili per valutare le vie di segnalazione coinvolte.
Tags
Cell DeathCell-free SupernatantProbioticsThree-dimensional SpheroidColorectal CancerLactobacillusAnti-cancer EffectIn Vivo MimicryMRS AgarAnaerobic ChamberMRS BrothL-cysteinePBSShaking IncubatorOD620CentrifugationRPMI 1640Fetal Bovine Serum0.22 Micron StrainerColorectal Cancer Cell LinesMonolayersTrypsin-EDTAGrowth Medium
