Journal
/
/
在结肠直肠癌细胞的三维球体培养物中使用无细胞益生菌超常剂评估细胞死亡
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
DOI:

06:07 min

June 13, 2020

, , , ,

Chapters

  • 00:04Introduction
  • 00:34Bacterial Cell Culture and Lactobacillus Cell-Free Supernatant (LCFS) Preparation
  • 02:18Colorectal Cancer Cell Line Preparation
  • 03:12Spheroid Formation
  • 03:493D colorectal Cancer Cell LCFS Treatment
  • 04:26Results: Representative Effects of LCFS Co-Culture on Spheroid Formation and Viability
  • 05:22Conclusion

Summary

Automatic Translation

这里介绍了了解 乳酸杆菌 无细胞超高分子(LCFS)的抗癌作用的方法。结肠直肠癌细胞系显示在3D培养物中用LCFS治疗时细胞死亡。生成球体的过程可以根据脚手架进行优化,并且所呈现的分析方法可用于评估所涉及的信号通路。

Related Videos

Read Article