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Cancer Research
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大鼠乳腺内皮细胞移植到间膜白脂肪垫
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Cancer Research
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JoVE Journal
Cancer Research
Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
Please note that all translations are automatically generated.
Click here for the English version.
大鼠乳腺内皮细胞移植到间膜白脂肪垫
DOI:
10.3791/60401-v
•
10:41 min
•
March 04, 2020
•
Lauren B. Shunkwiler
,
Jill D. Haag
,
Michael N. Gould
,
Bart M. G. Smits
1
Department of Pathology and Laboratory Medicine
,
Medical University of South Carolina
,
2
Department of Oncology, McArdle Laboratory for Cancer Research
,
University of Wisconsin-Madison School of Medicine and Public Health
Chapters
00:05
Introduction
00:44
Harvesting Donor Rat Mammary Gland Epithelium
01:35
Extract Brain Tissue from Euthanized Donors
03:04
Digestion and Processing of Mammary Gland Extracts
05:08
Transplantation Procedure (Recipient Rat 4-5 Weeks of Age)
06:53
Assessment of Epithelial Outgrowth
07:40
Results: Cell Transplantation into the Interscapular White Fat Pad
09:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种移植方法,将供体大鼠乳腺上皮细胞移植到受体动物的细胞间白脂肪垫中。该方法可用于检查宿主和/或供体对乳腺上皮发育的影响,并消除预清除的需要,从而扩展了此技术的实用性。
Tags
Rat Mammary Epithelial Cell
Cell Transplantation
Interscapular White Fat Pad
Breast Cancer Susceptibility Research
Mammary Cell Autonomous
Host Effects
Endogenous Membrane Epithelium
Euthanized Female Rat
Serum-free DMEM F12 Media
Cell Transplantation Assays
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