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Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
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1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences,Universiti Putra Malaysia, 2Institute of Tropical Forestry and Forestry Products (INTROP),Universiti Putra Malaysia, 3Department of Biomedical Science, Faculty of Medicine,University of Malaya, 4Agro-Biotechnology Institute (ABI),National Institute of Biotechnology Malaysia (NIBM)
Chapters
- 00:00Title
- 00:53Live Cells Co-Culture Model of 4T1 Mouse Mammary Carcinomas Cells and Raw 264.7 Mouse Macrophages
- 06:52Time-lapse Assessment of M1 Macrophages Phagocyted 4T1 Cells
- 08:18Results
- 09:49Conclusion
A atividade fagocítica do macrófago contra as células cancerosas, especificamente as células carcinomas mamárias do camundongo 4T1, foi imagemda neste estudo. O modelo de cocultura de células vivas foi estabelecido e observado por meio de uma combinação de microscopia de contraste de interferência fluorescente e diferencial. Esta avaliação foi imagemdo usando o software de imagem para desenvolver multiponto de vídeo time-lapse.
