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M1巨噬细胞-4T1小鼠乳腺癌细胞在共培养中噬菌体活性的时移2D成像
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Cancer Research
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JoVE Journal Cancer Research
Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
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M1巨噬细胞-4T1小鼠乳腺癌细胞在共培养中噬菌体活性的时移2D成像

DOI:
10.3791/60281-v

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10:23 min

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December 14, 2019

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, , , , , ,
1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences,Universiti Putra Malaysia, 2Institute of Tropical Forestry and Forestry Products (INTROP),Universiti Putra Malaysia, 3Department of Biomedical Science, Faculty of Medicine,University of Malaya, 4Agro-Biotechnology Institute (ABI),National Institute of Biotechnology Malaysia (NIBM)

Chapters

  • 00:00Title
  • 00:53Live Cells Co-Culture Model of 4T1 Mouse Mammary Carcinomas Cells and Raw 264.7 Mouse Macrophages
  • 06:52Time-lapse Assessment of M1 Macrophages Phagocyted 4T1 Cells
  • 08:18Results
  • 09:49Conclusion

Summary

Automatic Translation

Automatic Translation

在这项研究中，对癌细胞，特别是4T1小鼠乳腺癌细胞的巨噬细胞噬菌体活性，被成像。利用荧光和差分干扰对比显微镜的组合，建立了活细胞共培养模型并观察了该模型。此评估使用成像软件进行成像，以开发多点延时视频。

Tags

Time-lapse2D ImagingPhagocytic ActivityM1 Macrophage4T1 Mouse Mammary CarcinomaCo-cultureFluorescent MicroscopyDIC MicroscopyPhagocytosisLive Cell ImagingCell CultureCell PolarizationRAW 264.7 MacrophagesLPSIFN-gamma

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