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Obtenção de esferas de células-tronco cancerígenas de tumores ginecológicos e de câncer de mama
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1Institute of Biophysics, Faculty of Medicine,University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine,University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine,University of Coimbra, 6Gynecology A Service,Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine,University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service,Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School,Laboratory Biomedical Sciences
Chapters
- 00:05Introduction
- 00:35Sphere Formation and Derived Adherent Population Generation
- 02:28Sphere-forming Capacity Determination
- 03:02Self-renewal Determination
- 03:59Sphere Projection Area Determination
- 04:51Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
- 06:29Conclusion
O objetivo dessa metodologia é identificar células-tronco cancerígenas (CSC) em linhas de células cancerosas e amostras primárias de tumor humano com o protocolo de formação da esfera, de forma robusta, utilizando ensaios funcionais e caracterização phenotípica com citometria de fluxo e ocidental Borrão.
