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Obtenir des sphères de cellules souches cancéreuses à partir de tumeurs gynécologiques et du cancer du sein
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1Institute of Biophysics, Faculty of Medicine,University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine,University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine,University of Coimbra, 6Gynecology A Service,Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine,University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service,Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School,Laboratory Biomedical Sciences
Chapters
- 00:05Introduction
- 00:35Sphere Formation and Derived Adherent Population Generation
- 02:28Sphere-forming Capacity Determination
- 03:02Self-renewal Determination
- 03:59Sphere Projection Area Determination
- 04:51Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
- 06:29Conclusion
L’objectif de cette méthodologie est d’identifier les cellules souches cancéreuses (CSC) dans les lignées de cellules cancéreuses et les échantillons primaires de tumeurs humaines avec le protocole de formation de sphère, d’une manière robuste, en utilisant des essais fonctionnels et la caractérisation phénotypique avec cytométrie du flux et de l’Ouest Tache.
