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使用锚定多路聚合酶链反应的致癌基因融合检测,随后进行下一代测序
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JoVE Journal
Cancer Research
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
使用锚定多路聚合酶链反应的致癌基因融合检测,随后进行下一代测序
DOI:
10.3791/59895-v
•
09:49 min
•
July 05, 2019
•
Michael Seager
,
Dara L. Aisner
,
Kurtis D. Davies
1
Department of Pathology
,
University of Colorado – Anschutz Medical Campus
Chapters
00:04
Title
00:49
Random Priming and First Strand cDNA Synthesis
01:55
Ligation Step 2 and Bead Purification
04:25
First PCR and Bead Purification
05:48
Library Quantitation and Sequencing
07:41
Assay Data Interpretation
08:39
Results: Sample Summary and Fusion Calls
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文详细介绍了使用锚定多路聚合酶链式反应基库制备试剂盒,然后进行下一代测序,以评估临床固体肿瘤样本中的致癌基因融合。介绍了湿凳和数据分析步骤。
Tags
Gene Fusion Detection
Anchored Multiplex PCR
Next-generation Sequencing
Oncogenic Gene Fusions
Tumor Samples
Formulative Fixation
Diagnosis
Prognosis
Treatment Selection
Artifacts
Random Priming
First Strand CDNA Synthesis
Second Strand CDNA Synthesis
Bead Purification
Molecular Barcode Adapter
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