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Immunology and Infection
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巨型囊泡内单细胞层的细菌细胞培养
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
Please note that all translations are automatically generated.
Click here for the English version.
巨型囊泡内单细胞层的细菌细胞培养
DOI:
10.3791/59555-v
•
07:33 min
•
April 30, 2019
•
Masamune Morita
,
Yuri Ota
2
,
Kaoru Katoh
,
Naohiro Noda
2
1
Biomedical Research Institute
,
National Institute of Advanced Industrial Science and Technology (AIST)
,
2
Department of Life Science and Medical Bioscience
,
Waseda University
Chapters
00:04
Title
00:31
Lipid-containing Oil Solution and Small Vesicle (SV) Preparation
01:54
Bacterial Cell Pre-culture
02:45
Giant Vesicle (GV) and Water-in-Oil (W/O) Bacterial Cell-containing Droplet Formation
04:11
Handmade Chamber and Supported Bilayer Membrane Preparation
04:54
GV Immobilization and Microscopic Bacterial Growth Observation
06:04
Results: Representative GV Containing Bacteria and Bacterial Growth Evaluation
06:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们演示了巨型囊泡 (GV) 内的细菌的单细胞培养。含有细菌细胞的GV通过滴转移方法制备,固定在玻璃基板上的支撑膜上,直接观察细菌生长。这种方法也可以适应其他细胞。
Tags
Bacterial Cell Culture
Single-cell Level
Giant Vesicles
POPC
Biotin-PEG-DSPE
Mineral Oil
Sonication
Extrusion
E. Coli
LB Medium
Optical Density
Sucrose Solution
Outer Aqueous Solution
Lipid-containing Oil Solution
Water-oil Droplet Formation
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