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Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

DOI:
10.3791/61376-v

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08:33 min

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August 05, 2020

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, ,
1Department of Microbiology and Immunology, Carver College of Medicine,University of Iowa

Chapters

  • 00:04Introduction
  • 00:34Preparation of Agarose Pads
  • 01:28Equilibrating Materials to Experimental Temperature
  • 02:06Inoculation of Agarose Pads
  • 03:13Microscope Setup for Live Imaging
  • 05:32Results: P. aeruginosa Behavior when Cocultured with S. aureus
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

This live-bacterial cell imaging protocol allows for visualization of interactions between multiple bacterial species at the single-cell level over time. Time-lapse imaging allows for observation of each bacterial species in monoculture or coculture to interrogate interspecies interactions in multispecies bacterial communities, including individual cell motility and viability.

Tags

Single-cellInterspeciesBacterial InteractionsKinetic VisualizationAgarose PadsCell TrackingGene ExpressionViability StainingBacterial Growth MediaEnvironmental ChamberHumidity WipesCo-cultureMicroscopy

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