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1Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine,Southeast University, 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering,Southeast University
Chapters
- 00:04Title
- 01:00Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
- 03:52PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
- 05:00In Vitro RAW264.7 Macrophages Transfection
- 06:55Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
- 08:51Conclusion
Descriviamo un metodo di utilizzo polyethyleneimine (PEI)-rivestito di nanoparticelle di ossido di ferro superparamagnetico per macrofagi trasfezione con siRNA. Queste nanoparticelle è in grado di fornire in modo efficiente siRNA di espressione del gene bersaglio macrofagi in vitro e in vivo e silenzio.
