JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
聚乙胺包覆氧化铁纳米粒子作为
载体, 在体外
和
体内向
巨噬细胞输送小干扰 rna
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages
In Vitro
and
In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
聚乙胺包覆氧化铁纳米粒子作为
载体, 在体外
和
体内向
巨噬细胞输送小干扰 rna
DOI:
10.3791/58660-v
•
09:36 min
•
February 05, 2019
•
Na Jia*
1
,
Haoan Wu*
2
,
Juanli Duan
,
Chunxue Wei
,
Kaixuan Wang
,
Yu Zhang
,
Xiaohua Mao
1
Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine
,
Southeast University
,
2
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering
,
Southeast University
Chapters
00:04
Title
01:00
Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
03:52
PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
05:00
In Vitro RAW264.7 Macrophages Transfection
06:55
Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
08:51
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们描述了一种使用聚乙烯烯胺 (pei) 涂层超顺磁性氧化铁纳米粒子与 sirna 转染巨噬细胞的方法。这些纳米颗粒可以有效地传递 sirna 巨噬细胞在
体外
和
体内
和沉默目标基因表达。
Tags
Polyethyleneimine
Iron Oxide Nanoparticles
SPION
Macrophage
Delivery
SiRNA
In Vitro
In Vivo
Allylic Acid
Dimercapto Acrylic Acid
Triethylamine
PH
Nanoparticle Synthesis
Coating
Inflammation
Cancer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
一项战略,以确定在常见的疾病,如自闭症和精神分裂症的新突变
脑Microslices的兴奋性刺激作为<em>在体外</em>中风模型
通过40W的Elvax缓释给药的大鼠视网膜:对慢性疾病的治疗
隔离和使用的牛肠系膜动脉和静脉部分,作为生物测定在小肠测试的Vasoactivity
宫内遥测来衡量鼠标收缩压力<em>在体内</em
从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析
优化分析<em>在体内</em>和<em>体外</em>脂肪肝
体外
和
体内
检测人类细胞中的 Mitophagy,
线虫
和小鼠
在体内
MRI 对小鼠股骨骨愈合过程中骨折愈伤组织发育的评价
一项临床试验,评估基于橄榄油的三腔袋的亲子营养的安全性、有效性和交付
Read Article