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Journal
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Immunology and Infection
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イムノフェノ タイプと大量のフローサイトメトリーによる末梢血中のサイトカイン産生の単一細胞解析
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
イムノフェノ タイプと大量のフローサイトメトリーによる末梢血中のサイトカイン産生の単一細胞解析
DOI:
10.3791/57780-v
•
12:36 min
•
June 26, 2018
•
Ryan M. Baxter*
1
,
Daniel S. Kong*
1
,
Josselyn E. Garcia-Perez
,
William E. O’Gorman
,
Elena W.Y. Hsieh
3
1
Department of Immunology and Microbiology
,
University of Colorado School of Medicine
,
2
OMNI Biomarkers, Development Sciences
,
Genentech
,
3
Department of Pediatrics, Division of Allergy and Immunology
,
University of Colorado School of Medicine
Chapters
00:04
Title
01:10
Stimulation and Processing of Peripheral Whole Blood
05:27
Barcode of Lysed/Fixed Blood Cells
09:44
Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
11:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
免疫表現型と機能 (細胞内サイトカイン誘導) を評価する単一細胞プロテオミクスの手法をここで述べる末梢全血サンプル、大量のフローサイトメトリーによる分析の変化。
Tags
Single-cell Analysis
Immunophenotype
Cytokine Production
Peripheral Whole Blood
Mass Cytometry
Autoimmunity
Cell Frequency
Cytokine Production
Systems Immunology
Autoimmune Disease
SLE
Whole Blood Processing
Ruxolitinib
Lyse/fix Buffer
Cell Recovery
Antibody Staining
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