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Eencellige analyse van Immunophenotype en Cytokine productie in perifere bloed via massale Cytometry
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
DOI:

12:36 min

June 26, 2018

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Chapters

  • 00:04Title
  • 01:10Stimulation and Processing of Peripheral Whole Blood
  • 05:27Barcode of Lysed/Fixed Blood Cells
  • 09:44Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
  • 11:50Conclusion

Summary

Automatic Translation

Hier beschrijven we een eencellige proteomic aanpak om te evalueren van immuun fenotypische en functionele (intracellulaire cytokine inductie) veranderingen in het perifere bloedmonsters geanalyseerd via massale cytometry.

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