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Journal
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Immunology and Infection
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大鼠外周全血细胞免疫表型和因子生成的单细胞分析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
大鼠外周全血细胞免疫表型和因子生成的单细胞分析
DOI:
10.3791/57780-v
•
12:36 min
•
June 26, 2018
•
Ryan M. Baxter*
1
,
Daniel S. Kong*
1
,
Josselyn E. Garcia-Perez
,
William E. O’Gorman
,
Elena W.Y. Hsieh
3
1
Department of Immunology and Microbiology
,
University of Colorado School of Medicine
,
2
OMNI Biomarkers, Development Sciences
,
Genentech
,
3
Department of Pediatrics, Division of Allergy and Immunology
,
University of Colorado School of Medicine
Chapters
00:04
Title
01:10
Stimulation and Processing of Peripheral Whole Blood
05:27
Barcode of Lysed/Fixed Blood Cells
09:44
Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
11:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一个单细胞蛋白质组学方法, 以评估免疫表型和功能 (细胞因子诱导) 改变周围全血标本, 分析通过大规模细胞术。
Tags
Single-cell Analysis
Immunophenotype
Cytokine Production
Peripheral Whole Blood
Mass Cytometry
Autoimmunity
Cell Frequency
Cytokine Production
Systems Immunology
Autoimmune Disease
SLE
Whole Blood Processing
Ruxolitinib
Lyse/fix Buffer
Cell Recovery
Antibody Staining
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