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基于3D 荧光显微镜的病毒转录工厂功能成像
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Biology
Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
基于3D 荧光显微镜的病毒转录工厂功能成像
DOI:
10.3791/56832-v
•
09:03 min
•
January 18, 2018
•
Christopher P. Chen
,
Frank Chuang
,
Yoshihiro Izumiya
1
Department of Dermatology, University of California Davis School of Medicine
,
University of California, Davis
,
2
Center for Biophotonics, Department of Biochemistry and Molecular Medicine
,
University of California, Davis
Chapters
00:05
Title
00:39
Fixation and Permeabilization
03:08
IFA and RNA-FISH
06:59
Results: Viral Transcription Clearly Visible
08:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
病毒转录工厂是分散的结构, 富含细胞 RNA 聚合酶 II, 以增加病毒基因转录在重新激活。本文介绍了一种通过免疫荧光染色和
原位
RNA 杂交技术在3D 核空间中主动转录病毒染色质位置的方法。
Tags
Viral Transcription Factories
3D Fluorescence Microscopy
Nascent RNA-FISH
Amino Acid Staining
Viral Latent Proteins
Genomic Architectural Alterations
Inducible Viral Mini-chromosome Model
Cell Fixation
Cell Permeabilization
Primary Antibody Labeling
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