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1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg, 2Kidney Control of Homeostasis,Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg
Chapters
- 00:05Title
- 00:52Preparing the Organ Bath System
- 02:01Isolation of Mouse Aortas
- 03:03DHE and DAF-2DA Staining
- 04:47En Face Mounting and Confocal Microscopic Imaging
- 06:42Results: eNOS-uncoupling in Obese Mice
- 07:56Conclusion
En este artículo se describe un método relativamente fácil adaptarse usando el colorante fluorescente diaminofluorescein-2 diacetato (DAF-2DA) y dihydroethidium (DHE) para la detección simultánea en face y visualización de óxido nítrico intracelular (NO) y el anión superóxido (O 2 .- ), respectivamente, en las aortas recién aisladas intactos de un modelo de obesidad ratón.
