Journal
/
Biology
/
一氧化氮和超氧化物歧化酶恩的人脸检测在完整的动脉内皮细胞层
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Please note that all translations are automatically generated. Click here for the English version.

一氧化氮和超氧化物歧化酶恩的人脸检测在完整的动脉内皮细胞层

DOI:
10.3791/53718-v

•

08:58 min

•

February 25, 2016

•

, , , ,
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg, 2Kidney Control of Homeostasis,Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg

Chapters

  • 00:05Title
  • 00:52Preparing the Organ Bath System
  • 02:01Isolation of Mouse Aortas
  • 03:03DHE and DAF-2DA Staining
  • 04:47En Face Mounting and Confocal Microscopic Imaging
  • 06:42Results: eNOS-uncoupling in Obese Mice
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

在这篇文章中我们将介绍使用荧光染料diaminofluorescein-2双乙酸钠（DAF-2DA）和二氢乙啶（DHE）对恩面对同时检测和细胞内一氧化氮的可视化（NO）和超氧阴离子（O 2的调整相对容易的方法.- ）分别在新鲜分离的肥胖小鼠模型中的完整主动脉。

Tags

Nitric OxideSuperoxideEndothelial LayerIntact ArteriesEn Face DetectionDAF-2DADHECardiovascular PhysiologyEndothelial DysfunctionENOS UncouplingOrgan Bath SystemKrebs-Ringer Bicarbonate BufferAortic RingsDissectionFluorescent Staining

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

检测采用ELISA法的母语完整的核小体的翻译后修饰

从细胞中一氧化氮和超氧负离子自由基的电子顺磁共振光谱的检测，使用自旋陷阱

从鼠标阻力动脉分离微血管内皮管

单线粒体超氧闪烁在完整心脏或共聚焦成像<em>在体内</em

在斑马鱼胚胎氧化应激分析

细胞内钙的双光子成像<sup> 2+</sup>的离体大鼠主动脉的处理和一氧化氮的内皮细胞和平滑肌细胞

发展与表征<em>体外</em>微血管网络和内皮的定量测量的[Ca<sup> 2+</sup>]<sub>我</sub>和一氧化氮含量

用化学发光法测定硝酸盐和亚硝酸盐，代谢产物中的一氧化氮途径，在生物材料

面对小鼠血液制备

遗传编码的荧光一氧化氮的应用（NO•）探针，所述geNOps，用于在单细胞NO•信号的实时影像

Read Article