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Chapters
- 00:05Title
- 02:13Protein Extraction
- 03:03Endoproteinase LysC and Tryptic Protein Digestion of Whole Lysate Using Filter Assisted Sample Preparation
- 05:34Ion Exchange Chromatography
- 06:20GELFREE Fractionation of Cochlear Sensory Epithelium
- 07:54Protein Digestion of GELFREE Fractions Using Filter Assisted Sample Preparation
- 10:07Results: Identification of membrane and Soluble Proteins by High-resolution Mass Spectrometry Analysis
- 14:00Conclusion
