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建立浮星细胞（MBC-P）和生物膜细胞（MBC-B）抗菌剂的最小杀菌浓度

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Immunology and Infection

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JoVE Journal Immunology and Infection

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

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建立浮星细胞（MBC-P）和生物膜细胞（MBC-B）抗菌剂的最小杀菌浓度

DOI:

10.3791/50854-v

•

06:36 min

•

January 02, 2014

•

Thien-Fah Mah

¹Department of Biochemistry, Microbiology and Immunology,University of Ottawa

Chapters

00:05Title
01:03Minimal Bactericidal Concentration of Biofilm Cells (MBC-B)
02:40Assay for Live Cells
03:34Minimal Bactericidal Concentration of Planktonic Cells (MBC-P)
05:22Results: MBC-P and MBC-B Assays
06:18Conclusions

Summary

Automatic Translation

该协议允许使用 96 井微蒂板直接比较浮石和生物膜耐药性，以识别细菌菌株 ，从而在体外 形成生物膜。浮游生物或生物膜细菌暴露在首选抗菌剂的连续稀释中。活力被阿加板块的生长所测定。

Tags

Minimal Bactericidal Concentration MBC Antimicrobial Agent Planktonic Cells MBC-P Biofilm Cells MBC-B Bacterial Strain Microtiter Plate Antimicrobial Resistance Biofilm Formation Biofilm Recovery Cell Viability Assay

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