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Chapters
- 00:00Title
- 00:13Introduction
- 01:07Two Techniques for the Orthotopic Mouse Model of Colorectal Cancer
- 01:50Instruments
- 02:49Mouse Preparation, Laparotomy, and Exposure of Cecum
- 04:37Injection of Cells into Cecal Wall
- 05:07Transplantation of Tumor onto Cecum
Duas técnicas podem ser usadas para estabelecer esse modelo: injeção de uma suspensão de células de câncer na parede cecal ou transplante de um pedaço de tumor subcutâneo para o ceco. Este modelo é útil para estudar a progressão natural do câncer colo-retal e testes de novos agentes terapêuticos contra o câncer colorretal.
Tags
Orthotopic Mouse ModelColorectal CancerSubcutaneous Tumor ModelCancer CellsNatural LocationHuman DiseaseHigh FidelityTechniquesMouse AnesthesiaLaparotomyCecumColorectal Cancer Cell SuspensionSingle Cell SuspensionInjectionLeakageTransplantationSubcutaneous TumorEuthanizedSterile TechniqueTumor PieceTumor Cell InfiltrationPrimary TumorsLiver MetastasesTherapeutic Agents
