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Chapters
- 00:00Creating channel inlet and outlet
- 00:06Introduction
- 01:27Microfabrication of microfluidic device
- 01:45Preparation of PDMS mixture
- 02:42Patterning PDMS molds
- 03:36Removing bubbles by vacuum
- 04:21Assembly of microfluidic device
- 04:55Cutting layers of microfluidic device
- 06:10Bonding the microfluidic device layers
- 08:19Coating channels with fibronectin
- 09:08Experiment: monitoring apoptosis
- 09:09Cell seeding
- 11:31Application of reagents
- 13:32Time lapse of apoptosis
Nós descrevemos um protocolo para a fabricação de dispositivos microfluídicos que pode permitir a captura de células e cultura. Nesta abordagem padronizada microestruturas, tais como sulcos dentro de canais microfluídicos são usados para criar regiões de cisalhamento de baixa tensão dentro do qual célula pode encaixar.
Tags
Microfluidic DeviceGroove PatternsCellular BehaviorMicrogrooved SubstrateFabricationPoly(dimethylsiloxane) (PDMS)Mouse Fibroblast CellsApoptosisOxidative StressAnnexin VPropidium IodidePhosphatidylserinePlasma MembraneReal-time ObservationInverted MicroscopeIncubation ChamberHigh-throughput Drug Screening
