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微流体装置与Groove的模式,为研究细胞行为
JoVE Journal
Biology
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JoVE Journal
Biology
A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
Please note that all translations are automatically generated.
Click here for the English version.
微流体装置与Groove的模式,为研究细胞行为
DOI:
10.3791/270-v
•
13:50 min
•
August 30, 2007
•
Bong Geun Chung
,
Amir Manbachi
,
Ali Khademhosseini
1
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine
,
Brigham and Women’s Hospital
Chapters
00:00
Creating channel inlet and outlet
00:06
Introduction
01:27
Microfabrication of microfluidic device
01:45
Preparation of PDMS mixture
02:42
Patterning PDMS molds
03:36
Removing bubbles by vacuum
04:21
Assembly of microfluidic device
04:55
Cutting layers of microfluidic device
06:10
Bonding the microfluidic device layers
08:19
Coating channels with fibronectin
09:08
Experiment: monitoring apoptosis
09:09
Cell seeding
11:31
Application of reagents
13:32
Time lapse of apoptosis
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种用于制造微流体装置,可以使细胞的捕获和文化协议。在这种方法中,如微流体通道内的沟槽图案的微观结构是用来建立低剪切应力区域内的细胞可以停靠。
Tags
Microfluidic Device
Groove Patterns
Cellular Behavior
Microgrooved Substrate
Fabrication
Poly(dimethylsiloxane) (PDMS)
Mouse Fibroblast Cells
Apoptosis
Oxidative Stress
Annexin V
Propidium Iodide
Phosphatidylserine
Plasma Membrane
Real-time Observation
Inverted Microscope
Incubation Chamber
High-throughput Drug Screening
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