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Chapters
- 00:00Creating channel inlet and outlet
- 00:06Introduction
- 01:27Microfabrication of microfluidic device
- 01:45Preparation of PDMS mixture
- 02:42Patterning PDMS molds
- 03:36Removing bubbles by vacuum
- 04:21Assembly of microfluidic device
- 04:55Cutting layers of microfluidic device
- 06:10Bonding the microfluidic device layers
- 08:19Coating channels with fibronectin
- 09:08Experiment: monitoring apoptosis
- 09:09Cell seeding
- 11:31Application of reagents
- 13:32Time lapse of apoptosis
Abbiamo descritto un protocollo per la fabbricazione di dispositivi microfluidici che può consentire la cattura delle cellule e della cultura. In questo approccio microstrutture modellata come scanalature all'interno dei canali microfluidica sono utilizzati per creare le regioni a basso sforzo di taglio all'interno del quale cellula possono attraccare.
Tags
Microfluidic DeviceGroove PatternsCellular BehaviorMicrogrooved SubstrateFabricationPoly(dimethylsiloxane) (PDMS)Mouse Fibroblast CellsApoptosisOxidative StressAnnexin VPropidium IodidePhosphatidylserinePlasma MembraneReal-time ObservationInverted MicroscopeIncubation ChamberHigh-throughput Drug Screening
