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检测信号效应复合物BMP4的下游使用原位解放军,接近结扎分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
DOI:

12:52 min

March 03, 2011

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Chapters

  • 00:00Title
  • 02:56Plating of Cells and Treatment with BMP
  • 04:12Fixation and Permeabilization
  • 05:26Blocking and Primary Antibodies Application
  • 06:49The Proximity Ligation Assay (PLA)
  • 09:54Preparation for Imaging
  • 10:56In Situ Proximity Ligation Assay Results from Neuro2a Cells Treated with BMP4
  • 12:18Conclusion

Summary

Automatic Translation

在这里,我们展示了如何使用接近结扎含量(解放军),与抗体结合的可视化骨形态发生蛋白(BMP),在固定的细胞信号。这种技术允许我们遵循激活效应的复合物的内源性BMP的核积累和BMP4的刺激下,他们的水平随着时间的推移进行量化。

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